Piovan, C, Palmieri, D, Di Leva, G, Braccioli, L, Casalini, P, Nuovo, G, Tortoreto, M, Sasso, M, Plantamura, I, Triulzi, T, Taccioli, C, Tagliabue, E, Iorio, MV and Croce, CM (2012) Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer. Molecular Oncology, 6 (4). 458 - 472. ISSN 1574-7891

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An increasing body of evidence highlights an intriguing interaction between microRNAs and transcriptional factors involved in determining cell fate, including the well known "genome guardian" p53. Here we show that miR-205, oncosuppressive microRNA lost in breast cancer, is directly transactivated by oncosuppressor p53. Moreover, evaluating miR-205 expression in a panel of cell lines belonging to the highly aggressive triple negative breast cancer (TNBC) subtype, which still lacks an effective targeted therapy and characterized by an extremely undifferentiated and mesenchymal phenotype, we demonstrated that this microRNA is critically down-expressed compared to a normal-like cell line. Re-expression of miR-205 where absent strongly reduces cell proliferation, cell cycle progression and clonogenic potential in vitro, and inhibits tumor growth in vivo, and this tumor suppressor activity is at least partially exerted through targeting of E2F1, master regulator of cell cycle progression, and LAMC1, component of extracellular matrix involved in cell adhesion, proliferation and migration.

Item Type: Article
Uncontrolled Keywords: miR-205; p53; E2F1; LAMC1
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Faculty of Medicine and Health Sciences > Institute for Science and Technology in Medicine
Related URLs:
Depositing User: Symplectic
Date Deposited: 30 Apr 2019 09:42
Last Modified: 03 Mar 2021 10:43
URI: https://eprints.keele.ac.uk/id/eprint/6243

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