Wenwu Li w.li@keele.ac.uk
Palmatine from Unexplored Rutidea parviflora Showed Cytotoxicity and Induction of Apoptosis in Human Ovarian Cancer Cells
Li, W; Johnson-Ajinwo, O; Richardson, A
Abstract
Ovarian cancer ranks amongst the deadliest cancers in the gynaecological category of cancers. This research work aims to evaluate in vitro anti-ovarian cancer activities and identify phytochemical constituents of a rarely explored plant speciesRutidea parviflora DC. The aqueous and organic extracts of the plant were evaluated for cytotoxicity using sulforhodamine B assay in four ovarian cancer cell lines and an immortalized human ovarian epithelial (HOE) cell line. The bioactive compounds were isolated and characterized by gas/liquid chromatography mass spectrometry and nuclear magnetic resonance spectroscopy. Caspase 3/7 activity assay, western blotting and flow cytometry were carried out to assess apoptotic effects of active compounds. The extracts/fractions of R. parviflora showed promising anti-ovarian cancer activities in ovarian cancer cell lines. A principal cytotoxic alkaloid was identified as palmatine whose IC50 was determined as 5.5-7.9 mu M. Palmatine was relatively selective towards cancer cells as it was less cytotoxic toward HOE cells, also demonstrating interestingly absence of cross-resistance in cisplatin-resistant A2780 cells. Palmatine further induced apoptosis by increasing caspase 3/7 activity, poly-ADP-ribose polymerase cleavage, and annexin V and propidium iodide staining in OVCAR-4 cancer cells. Our studies warranted further investigation of palmatine and R. parviflora extracts in preclinical models of ovarian cancer.
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 18, 2019 |
Publication Date | Apr 25, 2019 |
Journal | Toxins |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Pages | 237 - 237 |
DOI | https://doi.org/10.3390/toxins11040237 |
Keywords | Ovarian cancer; Rutidea parviflora; Palmatine; Apoptosis |
Publisher URL | https://www.mdpi.com/2072-6651/11/4/237 |
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