Stewart, KL, Hughes, E, Yates, EA, Akien, GR, Huang, T, Lima, M, Rudd, TR, Guerrini, M, Hung, S and Radford, SE (2016) Atomic details of the interactions of glycosaminoglycans with amyloid-β fibrils. Journal of the American Chemical Society, 138 (27). 8328 - 8331. ISSN 0002-7863

[thumbnail of M Lima - Atomic details of the interactions of glycisaminoglycans with amyloid-beta fibrils.pdf]
M Lima - Atomic details of the interactions of glycisaminoglycans with amyloid-beta fibrils.pdf - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview


The amyloid plaques associated with Alzheimer’s disease (AD) comprise fibrillar amyloid-β (Aβ) peptides as well as non-protein factors including glycosaminoglycan (GAG) polysaccharides. GAGs affect the kinetics and pathway of Aβ self-assembly and can impede fibril clearance; thus, they may be accessory molecules in AD. Here we report the first high-resolution details of GAG–Aβ fibril interactions from the perspective of the saccharide. Binding analysis indicated that the GAG proxy heparin has a remarkably high affinity for Aβ fibrils with 3-fold cross-sectional symmetry (3Q). Chemical synthesis of a uniformly 13C-labeled octasaccharide heparin analogue enabled magic-angle spinning solid-state NMR of the GAG bound to 3Q fibrils, and measurements of dynamics revealed a tight complex in which all saccharide residues are restrained without undergoing substantial conformational changes. Intramolecular 13C–15N dipolar dephasing is consistent with close (<5 Å) contact between GAG anomeric position(s) and one or more histidine residues in the fibrils. These data provide a detailed model for the interaction between 3Q-seeded Aβ40 fibrils and a major non-protein component of AD plaques, and they reveal that GAG–amyloid interactions display a range of affinities that critically depend on the precise details of the fibril architecture.

Item Type: Article
Additional Information: ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Subjects: Q Science > QH Natural history
Divisions: Faculty of Natural Sciences > School of Life Sciences
Depositing User: Symplectic
Date Deposited: 22 May 2019 08:37
Last Modified: 24 May 2019 10:09

Actions (login required)

View Item
View Item