Wu, F, Zhou, B, Wang, J, Zhong, M, Das, A, Watkinson, M, Hing, K, Zhang, D-W and Krause, S (2019) Photoelectrochemical Imaging System for the Mapping of Cell Surface Charges. Analytical Chemistry, 91 (9). 5896 -5903. ISSN 0003-2700

[thumbnail of single cell imaging with ITO -accepted.docx] Text
single cell imaging with ITO -accepted.docx - Accepted Version
Available under License Creative Commons Attribution Non-commercial.

Download (11MB)


The surface charge of cells affects cell signaling, cell metabolic processes, adherence to surfaces, and cell proliferation. Our understanding of the role of membrane charges is limited due to the inability to observe changes without interfering, chemically or physically, with the cell or its membrane. Here, we report that a photoelectrochemical imaging system (PEIS) based on label-free ac-photocurrent measurements at indium tin oxide (ITO) coated glass substrates can be used to map the basal surface charge of single live cells under physiological conditions. Cells were cultured on the ITO substrate. Photocurrent images were generated by scanning a focused, modulated laser beam across the back of the ITO coated glass substrate under an applied bias voltage. The photocurrent was shown to be sensitive to the negative surface charge of the substrate facing, basal side of a single living cell-an area not accessible to other electrochemical or electrophysiological imaging techniques. The PEIS was used to monitor the lysis of mesenchymal stem cells.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via American Chemical Society at http://doi.org/10.1021/acs.analchem.9b00304 - please refer to any applicable terms of use of the publisher.
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Natural Sciences > School of Chemical and Physical Sciences
Related URLs:
Depositing User: Symplectic
Date Deposited: 03 Jun 2019 14:51
Last Modified: 15 Apr 2020 01:30
URI: https://eprints.keele.ac.uk/id/eprint/6434

Actions (login required)

View Item
View Item