Sterchi, Erwin E (1978) Peptide hydrolases of the brush border membrane from human small intestine. Doctoral thesis, Keele University.

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A method has been developed for the purification of brush
border membranes from human small intestine. The method did not involve the use of EDTA-buffers or disruption of brush borders with high concentrations of Tris. On average, a 24-fold increase in specific activity for ot-giucosidase (brush border marker) was obtained in the final preparation which contained only traces of enzyme markers from other cellular organelles.
The homogenates of human small intestinal mucosa were shown
to contain enzymes capable of hydrolysing di-, tri- and oligopeptides as well as 2-naphthylamides. Distribution studies indicated that all of the oligopeptidase activity towards peptides, four and more amino
acids in length and activity towards leucine-2 -naphthylamide were
located exclusively in the brush border. A large proportion of activity towards a-glutamic acid-2-naphthylamide (aminopeptidase A),
y-glutamic acid-2 -naphthylamide (y-glutamyltransferase) and glycyl- proline-2 -naphthylamide (dipeptidyl peptidase IV) were also recovered
in the brush border membrane fraction. Depending on the substrate used, 33-87% of tripeptidase activity was located in the brush border membrane. An estimated 58-87% of dipeptidase activity, on the other hand, was recovered in the soluble fraction.
Solubilisation of brush border membrane proteins by sodium dodecyl sulphate, Triton X-100 and papain followed by polyacrylamide gel electrophoresis revealed seven different peptide hydrolases. These included the specific enzymes aminopeptidase A, dipeptidyl peptidase IV, y-glutamyltransferase and aminopeptidase M which were clearly separable on polyacrylamide gels after solubilisation with Triton X-100 or papain. Activity recovered in the aminopeptidase M peak in the above gel system could be resolved into two distinct peptidases in addition to aminopeptidase M, by SDS-gel electro­phoresis. One of these peptidases was most active towards aliphatic tripeptides (peptidase 1 ) while the other appeared to be specific for dipeptides. A further peptidase (aminopeptidase M’) was resolved by isoelectric polyacrylamide gels. The role of these brush border peptide hydrolases in the absorption of protein by the gut is discussed.

Item Type: Thesis (Doctoral)
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Natural Sciences > School of Life Sciences
Depositing User: Lisa Bailey
Date Deposited: 24 Sep 2019 14:53
Last Modified: 24 Sep 2019 14:53

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