Fang, L, Trigiante, G, Crespo-Otero, R, Philpott, MP, Jones, CR and Watkinson, M (2019) An alternative modular ‘click-SNAr-click’ approach to develop subcellular localised fluorescent probes to image mobile Zn2+. Organic & Biomolecular Chemistry, 17 (47). pp. 10013-10019. ISSN 1477-0520

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Zn2+ is involved in a number of biological processes and its wide-ranging roles at the subcellular level, especially in specific organelles, have not yet been fully established due to a lack of tools to image it effectively. We report a new and efficient modular double ‘click’ approach towards a range of sub-cellular localised probes for mobile zinc. Through this methodology, endoplasmic reticulum, mitochondria and lysosome localised probes were successfully prepared which show good fluorescence responses to mobile Zn2+ in vitro and in cellulo whilst a non-targeting probe was synthesized as a control. The methodology appears to have wide-utility for the generation of sub-cellular localised probes by incorporating specific organelle targeting vectors for mobile Zn2+ imaging.

Item Type: Article
Additional Information: This is the accepted author manuscript (AAM). The final published version (version of record) is available online via Royal Society of Chemistry at - please refer to any applicable terms of use of the publisher.
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Natural Sciences > School of Chemical and Physical Sciences
Depositing User: Symplectic
Date Deposited: 22 Oct 2019 14:39
Last Modified: 10 Oct 2020 01:30

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