Alekos Simoni
A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae
Simoni, Alekos; Hammond, Andrew M.; Beaghton, Andrea K.; Galizi, Roberto; Taxiarchi, Chrysanthi; Kyrou, Kyros; Meacci, Dario; Gribble, Matthew; Morselli, Giulia; Burt, Austin; Nolan, Tony; Crisanti, Andrea
Authors
Andrew M. Hammond
Andrea K. Beaghton
Roberto Galizi r.galizi@keele.ac.uk
Chrysanthi Taxiarchi
Kyros Kyrou
Dario Meacci
Matthew Gribble
Giulia Morselli
Austin Burt
Tony Nolan
Andrea Crisanti
Abstract
Only female insects transmit diseases such as malaria, dengue and Zika; therefore, control methods that bias the sex ratio of insect offspring have long been sought. Genetic elements such as sex-chromosome drives can distort sex ratios to produce unisex populations that eventually collapse, but the underlying molecular mechanisms are unknown. We report a male-biased sex-distorter gene drive (SDGD) in the human malaria vector Anopheles gambiae. We induced super-Mendelian inheritance of the X-chromosome-shredding I-PpoI nuclease by coupling this to a CRISPR-based gene drive inserted into a conserved sequence of the doublesex (dsx) gene. In modeling of invasion dynamics, SDGD was predicted to have a quicker impact on female mosquito populations than previously developed gene drives targeting female fertility. The SDGD at the dsx locus led to a male-only population from a 2.5% starting allelic frequency in 10-14 generations, with population collapse and no selection for resistance. Our results support the use of SDGD for malaria vector control.
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 1, 2020 |
Publication Date | 2020 |
Publicly Available Date | May 26, 2023 |
Journal | Nature Biotechnology |
Print ISSN | 1087-0156 |
Publisher | Nature Publishing Group |
ISBN | 15461696 10870156 |
DOI | https://doi.org/10.1038/s41587-020-0508-1 |
Keywords | Gene regulation; Genetics; Molecular engineering |
Publisher URL | https://doi.org/10.1038/s41587-020-0508-1 |
Files
s41587-020-0508-1.pdf
(2.1 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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