John R Glossop
DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS
Glossop, John R; Haworth, Kim E; Emes, Richard D; Nixon, Nicola B; Packham, Jon C; Dawes, Peter T; Fryer, Anthony A; Mattey, Derek L; Farrell, William E
Authors
Kim E Haworth
Richard D Emes
Nicola B Nixon
Jon C Packham
Peter T Dawes
Professor Anthony Fryer a.a.fryer@keele.ac.uk
Derek L Mattey
William E Farrell
Abstract
AIM: Alterations in DNA methylation contribute to the abnormal phenotype of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). We profiled genome-wide DNA methylation in these cells from synovial fluid, a more readily accessible source of disease-associated cells. PATIENTS & METHODS: Genome-wide DNA methylation was interrogated in fluid-derived FLS from five RA and six osteoarthritis patients using Human Methylation 450 Bead Chip and bisulfite pyrosequencing. RESULTS: Array analysis identified 328 CpGs, representing 195 genes, that were differentially methylated between RA and osteoarthritis fluid-derived FLS. Comparison with the genes identified in two independent studies of tissue-derived FLS revealed 73 genes in common (˜40%), of which 22 shared identity with both studies. Pyrosequencing confirmed altered methylation of these genes. CONCLUSION: Synovial fluid-derived RA FLS show methylation changes common with tissue-derived FLS, supporting the use of fluid-derived FLS for future investigations.
Journal Article Type | Article |
---|---|
Online Publication Date | Jun 25, 2015 |
Publication Date | Jun 1, 2015 |
Journal | Epigenomics |
Print ISSN | 1750-1911 |
Publisher | Future Medicine |
Peer Reviewed | Peer Reviewed |
Volume | 7 |
Issue | 4 |
Pages | 539 -551 |
DOI | https://doi.org/10.2217/epi.15.15 |
Keywords | DNA methylation, epigenetics, fibroblast-like synoviocyte, osteoarthritis, rheumatoid arthritis, synovial fluid |
Publisher URL | http://dx.doi.org/10.2217/epi.15.15 |
PMID | 26111028 |
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