Dowling, L, Roach, P, Rutter, A, Yousef, I, Pillai, S, Latham, D, van Pittius, DG and Sule-Suso, J (2020) EXPRESS: Optimization of Sample Preparation Using Glass Slides for Spectral Pathology. Applied Spectroscopy. 3702820945748 - ?. ISSN 1943-3530

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The clinical translation of Fourier transform infrared (FT-IR) microspectroscopy in pathology will require bringing this technique as close as possible to standard practice in pathology departments. An important step is sample preparation for both FT-IR microspectroscopy and pathology. This should entail minimal disruption of standard clinical practice while achieving good quality FT-IR spectral data. In fact, the recently described possibility of obtaining FT-IR spectra of cells placed on glass substrates brings FT-IR microspectroscopy closer to a clinical application. We have now furthered this work in order to identify two different types of lung cancer cells placed on glass coverslips. Two types of sample preparation which are widely used in pathology, cytospin and smear, have been used. Samples were fixed with either methanol, used in pathology, or formalin (4% paraformaldehyde) used widely in spectroscopy. Fixation with methanol (alcohol-based fixative) removed lipids from cells causing a decrease in intensity of the peaks at 2850 cm−1 and 2920 cm−1. Nevertheless, we show for the first time that using either type of sample preparation and fixation on thin glass coverslips allowed to differentiate between two different types of lung cancer cells using either the lipid region or the fingerprint region ranging from 1800 cm−1 to 1350 cm−1. We believe that formalin-fixed cytospin samples would be preferred to study cells on thin coverslips using FT-IR microspectroscopy. This work presents a clear indication for future advances in clinical assessment of samples within pathology units to gain a deeper understanding of cells/tissues under investigation.

Item Type: Article
Additional Information: The final version of this accepted manuscript can be found online with all relevant information at;
Uncontrolled Keywords: FTIR microspectroscopy, lung cancer, glass coverslips, cell fixation, cytospin, smear
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General) > R735 Medical education. Medical schools. Research
R Medicine > RB Pathology
R Medicine > RC Internal medicine
Divisions: Faculty of Medicine and Health Sciences > School of Pharmacy and Bioengineering
Related URLs:
Depositing User: Symplectic
Date Deposited: 29 Oct 2020 15:58
Last Modified: 08 Mar 2021 09:52

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