Meneghetti, MCZ, Deboni, P, Palomino, CMV, Braga, LP, Cavalheiro, RP, Viana, GM, Yates, EA, Nader, HB and Lima, MA (2021) ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis. Carbohydrate Polymers, 255. 117477 - 117477. ISSN 0144-8617

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The cell surface and extracellular matrix polysaccharide, heparan sulfate (HS) conveys chemical information to control crucial biological processes. HS chains are synthesized in a non-template driven process mainly in the Golgi apparatus, involving a large number of enzymes capable of subtly modifying its substitution pattern, hence, its interactions and biological effects. Changes in the localization of HS-modifying enzymes throughout the Golgi were found to correlate with changes in the structure of HS, rather than protein expression levels. Following BFA treatment, the HS-modifying enzymes localized preferentially in COPII vesicles and at the trans-Golgi. Shortly after heparin treatment, the HS-modifying enzyme moved from cis to trans-Golgi, which coincided with increased HS sulfation. Finally, it was shown that COPI subunits and Sec24 gene expression changed. Collectively, these findings demonstrate that knowledge of the ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the complete delineation of HS biosynthesis.

Item Type: Article
Additional Information: The final accepted version of this manuscript can be found online with all relevant information, including copyrights, at the following;
Uncontrolled Keywords: Biosynthesis; Heparan sulfate; COPI; COPII; Golgi apparatus
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
R Medicine > R Medicine (General) > R735 Medical education. Medical schools. Research
Divisions: Faculty of Natural Sciences > School of Life Sciences
Depositing User: Symplectic
Date Deposited: 16 Dec 2020 12:22
Last Modified: 03 Dec 2021 01:30

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