Wells, Tyler Robert (2021) Dopaminergic modulation of activity dependent synaptic plasticity in rodent lateral entorhinal cortex. Masters thesis, Keele University.

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The entorhinal cortex serves as the interface region between allocortical input and the hippocampus, with current theories suggesting that the region is involved in memory encoding, with dopamine serving as a “motivational currency” provided to memories of higher importance to the organism’s survival. This study aims to analyse the effects of dopamine on the ability to induce and maintain activity-dependent synaptic plasticity in the lateral entorhinal cortex. Here field recordings of excitatory post-synaptic potentials within rat lateral entorhinal cortex as well as bath application of drugs, including dopamine, were used to analyse their effects following stimulation of layers I and II of the lateral entorhinal cortex. The results indicate that the application of dopamine during paired-pulse low frequency stimulation causes a block of LTD. It was also found that the metaplastic effect of multiple dopamine applications of the same concentration remains in a slice which had been previously depressed by LTD. Results of LTP experiments were inconclusive, with a failure to induce LTP with either a high frequency stimulation or theta burst stimulation protocol. However, switching to a high calcium (10mM) ACSF during HFS improved the success rate of inducing LTP to 44%, with potentiation of the response remaining until the end of the 120-minute experiment. In conclusion, the results surrounding LTD provide further evidence to the theory that dopamine acts as a modulator of synaptic plasticity in the lateral entorhinal cortex (LEC). The LTP data requires further exploration, with use of a wider range of protocols to successfully induce LTP, at which point application of dopamine could be used to examine its effects.

Item Type: Thesis (Masters)
Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions: Faculty of Natural Sciences > School of Life Sciences
Contributors: Evans, Michael (Thesis advisor)
Caruana, Douglas (Thesis advisor)
Depositing User: Lisa Bailey
Date Deposited: 24 Mar 2021 10:00
Last Modified: 24 Mar 2021 10:00
URI: https://eprints.keele.ac.uk/id/eprint/9297

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