Citeroni, MR, Mauro, A, Ciardulli, MC, Di Mattia, M, El Khatib, M, Russo, V, Turriani, M, Santer, M, Della Porta, G, Maffulli, N, Forsyth, NR and Barboni, B (2021) Amnion-Derived Teno-Inductive Secretomes: A Novel Approach to Foster Tendon Differentiation and Regeneration in an Ovine Model. Frontiers in Bioengineering and Biotechnology, 9. 649288 - ?. ISSN 2296-4185

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Regenerative medicine has greatly progressed, but tendon regeneration mechanisms and robust in vitro tendon differentiation protocols remain to be elucidated. Recently, tendon explant co-culture (CO) has been proposed as an in vitro model to recapitulate the microenvironment driving tendon development and regeneration. Here, we explored standardized protocols for production and storage of bioactive tendon-derived secretomes with an evaluation of their teno-inductive effects on ovine amniotic epithelial cells (AECs). Teno-inductive soluble factors were released in culture-conditioned media (CM) only in response to active communication between tendon explants and stem cells (CMCO). Unsuccessful tenogenic differentiation in AECs was noted when exposed to CM collected from tendon explants (CMFT) only, whereas CMCO upregulated SCXB, COL I and TNMD transcripts, in AECs, alongside stimulation of the development of mature 3D tendon-like structures enriched in TNMD and COL I extracellular matrix proteins. Furthermore, although the tenogenic effect on AECs was partially inhibited by freezing CMCO, this effect could be recovered by application of an in vivo-like physiological oxygen (2% O2) environment during AECs tenogenesis. Therefore, CMCO can be considered as a waste tissue product with the potential to be used for the development of regenerative bio-inspired devices to innovate tissue engineering application to tendon differentiation and healing.

Item Type: Article
Additional Information: © 2021 Citeroni, Mauro, Ciardulli, Di Mattia, El Khatib, Russo, Turriani, Santer, Della Porta, Maffulli, Forsyth and Barboni. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Uncontrolled Keywords: amniotic stem cells, co-culture, conditioned media, tendon, tendon-differentiation, tissue engineering
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Related URLs:
Depositing User: Symplectic
Date Deposited: 07 Apr 2021 14:35
Last Modified: 07 Apr 2021 14:35

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