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Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions.

Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions. Thumbnail


Abstract

Carbohydrate-binding proteins from pathogenic bacteria and fungi have been shown to be implicated in various pathological processes, where they interact with glycans present on the surface of the host cells. These interactions are part of the initial processes of infection of the host and are very important to study at the atomic level. Here, we report the room temperature neutron structures of PLL lectin from Photorhabdus laumondii in its apo form and in complex with deuterated L-fucose, which is, to our knowledge, the first neutron structure of a carbohydrate-binding protein in complex with a fully deuterated carbohydrate ligand. A detailed structural analysis of the lectin-carbohydrate interactions provides information on the hydrogen bond network, the role of water molecules, and the extent of the CH-p stacking interactions between fucose and the aromatic amino acids in the binding site.

Acceptance Date Mar 3, 2021
Publication Date Mar 24, 2021
Publicly Available Date Mar 28, 2024
Journal Structure
Print ISSN 0969-2126
Publisher Elsevier
Pages 1003-1013
DOI https://doi.org/10.1016/j.str.2021.03.003
Publisher URL https://www.sciencedirect.com/science/article/pii/S0969212621000769?via%3Dihub

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