Hulme, CH, Fuller, HR, Riddell, J, Shirran, SL, Botting, CH, Osman, A and Wright, KT (2021) INVESTIGATION OF THE BLOOD PROTEOME IN RESPONSE TO SPINAL CORD INJURY IN RODENT MODELS. Spinal Cord. ISSN 1362-4393

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STUDY DESIGN: Explanatory and Mechanistic Study
OBJECTIVES: A better understanding of the ‘whole-body’ response following spinal cord injury (SCI) is needed to guide future research aimed at developing novel therapeutic interventions and identifying prognostic indicators for SCI. This study aimed to characterise the blood proteome following contusion or complete SCI compared to a sham injury in rat models.
SETTING: United Kingdom
METHODS: Pooled blood samples from one and seven days after a contusion (serum; n=5) or from 14 days and 112 days post-complete transection SCI (plasma; n=8) and their sham-injured counterparts were subjected to independent iTRAQ nanoflow liquid chromatography tandem mass spectrometry proteomic analyses. Pathway analyses of the proteins that were differentially abundant between SCI and their matched sham injured counterparts were completed to indicate biological pathways that may be changed in response to SCI.

RESULTS: Eleven and 42 proteins were differentially abundant (≥±2.0FC;p≤0.05) between the contusion SCI and sham injured animals at 24h and seven days post-injury, respectively. Seven and 12 proteins were differentially abundant between complete and sham injured rats at 14 and 112 days post-injury, respectively. Acute-phase response signalling and Liver X Receptor/Retinoic X Receptor activation were identified as differentially regulated pathways in both models of SCI.
We have utilised longitudinal preclinical SCI models to provide an insight into the blood proteome changes that result following SCI and to highlight a number of biological pathways of interest for future studies.

Item Type: Article
Additional Information: The final version of this article and all relevant information related to it, including copyrights, can be found online at the publishers website. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
Subjects: R Medicine > R Medicine (General)
R Medicine > R Medicine (General) > R735 Medical education. Medical schools. Research
R Medicine > RC Internal medicine
R Medicine > RC Internal medicine > RC925 Diseases of the musculoskeletal system
Divisions: Faculty of Medicine and Health Sciences > School of Pharmacy and Bioengineering
Depositing User: Symplectic
Date Deposited: 10 Sep 2021 09:45
Last Modified: 30 Nov 2021 15:11

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